The Magic Paintbrush is an item used by Shadow Mario in his attack on Isle Delfino in Super Mario Sunshine. It was used to paint goop all over the island, making the Shine Sprites flee. It is confirmed that Professor E. Gadd invented it, due to his logo on it.
The Brush is also the bat of Bowser Jr. in Mario Super Sluggers, and additionally used by Bowser Jr. in many other sports games.
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Bowser Jr. holding his Magic Paintbrush
Shadow Mario with Magic Paintbrush